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human proximal renal tubular epithelial cell line  (ATCC)


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    Structured Review

    ATCC human proximal renal tubular epithelial cell line
    Human Proximal Renal Tubular Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4553 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+renal+proximal+tubular+epithelial+cell+line+hk+2/HK-2/pm42262012-93-0-11
    Average 99 stars, based on 4553 article reviews
    human proximal renal tubular epithelial cell line - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: Protective effect of anisodamine hydrobromide on lipopolysaccharide-induced acute kidney injury
    Article Snippet: After centrifuged at 3500 g for 10 min at 4°C, the supernatant was used for the measurement of malondialdehyde (MDA) concentration (A003-1, Nanjing Jiancheng Bioengineering Institute, Jiangsu, China) and superoxide dismutase (SOD) activity (A001-1, Nanjing Jiancheng Bioengineering Institute, Jiangsu, China) using assay kits according to the manufacturer’s instructions, and IL-6 level (Neobioscience, Shenzhen, China) was determined using the ELISA kit. .. Human renal proximal tubular epithelial cell line HK-2 (ATCC, Rockville, Md, U.S.A.) were cultured in Dulbecco Modified Eagle medium (DMEM, Hyclone, U.S.A.) supplemented with 10% fetal bovine serum (Tianhang, Zhejiang, China) and 1% penicillin/streptomycin (Invitrogen, ThermoFisher, U.S.A.) at 37°C with 5% CO 2 . .. Cells were treated with or without a final concentration of 10 μg/ml AniHBr (Lot.190303, dissolved in ddH 2 O; Chengdu NO.1 Pharmaceutical Co., Ltd, China) in the presence of a final concentration of 10 ng/ml TNF-α (T6674, Sigma, U.S.A.) for 24 h. Then, the mito stress test was performed with the XF96 Extracellular Flux Analyzer (Seahorse Bioscience, MA, U.S.A.) according to the supper’s instructions [ ].

    Article Title: Suppression of lncRNA HOTAIR alleviates RCC angiogenesis through regulating miR-126/EGFL7 axis.
    Article Snippet: Renal cell carcinoma (RCC) has the highest mortality rate among urological cancers and tumor angiogenesis that plays a critical role in RCC progress.. Epidermal growth factor-like domain multiple 7 (EGFL7) has been recently identified as a regulator in RCC tumor angiogenesis and progression.. Long noncoding RNA (LncRNA) HOTAIR has been considered as a pro-oncogene in multiple cancers, but its precise mechanism of tumor angiogenesis has rarely been reported.

    Modification:

    Article Title: Protective effect of anisodamine hydrobromide on lipopolysaccharide-induced acute kidney injury
    Article Snippet: After centrifuged at 3500 g for 10 min at 4°C, the supernatant was used for the measurement of malondialdehyde (MDA) concentration (A003-1, Nanjing Jiancheng Bioengineering Institute, Jiangsu, China) and superoxide dismutase (SOD) activity (A001-1, Nanjing Jiancheng Bioengineering Institute, Jiangsu, China) using assay kits according to the manufacturer’s instructions, and IL-6 level (Neobioscience, Shenzhen, China) was determined using the ELISA kit. .. Human renal proximal tubular epithelial cell line HK-2 (ATCC, Rockville, Md, U.S.A.) were cultured in Dulbecco Modified Eagle medium (DMEM, Hyclone, U.S.A.) supplemented with 10% fetal bovine serum (Tianhang, Zhejiang, China) and 1% penicillin/streptomycin (Invitrogen, ThermoFisher, U.S.A.) at 37°C with 5% CO 2 . .. Cells were treated with or without a final concentration of 10 μg/ml AniHBr (Lot.190303, dissolved in ddH 2 O; Chengdu NO.1 Pharmaceutical Co., Ltd, China) in the presence of a final concentration of 10 ng/ml TNF-α (T6674, Sigma, U.S.A.) for 24 h. Then, the mito stress test was performed with the XF96 Extracellular Flux Analyzer (Seahorse Bioscience, MA, U.S.A.) according to the supper’s instructions [ ].

    Synthesized:

    Article Title: Suppression of lncRNA HOTAIR alleviates RCC angiogenesis through regulating miR-126/EGFL7 axis.
    Article Snippet: Renal cell carcinoma (RCC) has the highest mortality rate among urological cancers and tumor angiogenesis that plays a critical role in RCC progress.. Epidermal growth factor-like domain multiple 7 (EGFL7) has been recently identified as a regulator in RCC tumor angiogenesis and progression.. Long noncoding RNA (LncRNA) HOTAIR has been considered as a pro-oncogene in multiple cancers, but its precise mechanism of tumor angiogenesis has rarely been reported.



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